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Image Search Results
Journal: Phytomedicine : international journal of phytotherapy and phytopharmacology
Article Title: Poliumoside alleviates microglia-mediated inflammation and blood-brain barrier disruption via modulating the polarization of microglia after ischemic stroke in mice.
doi: 10.1016/j.phymed.2025.156881
Figure Lengend Snippet: Fig. 3. Pol attenuated microglial activation in vivo after stroke. (A-B). Representative images of Iba1 and CD16/32 (A) and CD206 (B) labeled brain tissue on day 1 after stroke; (D) Microglial endpoints/cell; (E) Process length/cell of microglia; the ratio of CD16/32+ (C) and CD206+ (F) cells(n = 4–5); (G-I) Western blot bands of iNOS, CD86, Arg-1 and CD206 in the brain on day 1 after stroke and β-actin internal control-based optical density quantification(n = 4–5). Bar= 20 μm. Data are presented as means ± SD, were performed by One-way ANOVA followed by Tukey’s multiple comparisons test. ^p < 0.05, ^^p < 0.01, ^^^p < 0.001, ^^^^p < 0.0001 tMCAO vs sham group; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 Pol-M vs tMCAO group; #p < 0.05, ##p < 0.01,### p < 0.001, ####p < 0.0001 Pol-H vs tMCAO group; $p < 0.05, $$p < 0.01, $$$ p < 0.001, $$$$p < 0.0001 EDB vs tMCAO group, ns = no statistical difference.
Article Snippet: The primary antibody used in this study was: rabbit anti
Techniques: Activation Assay, In Vivo, Labeling, Western Blot, Control
Journal: Phytomedicine : international journal of phytotherapy and phytopharmacology
Article Title: Poliumoside alleviates microglia-mediated inflammation and blood-brain barrier disruption via modulating the polarization of microglia after ischemic stroke in mice.
doi: 10.1016/j.phymed.2025.156881
Figure Lengend Snippet: Fig. 4. Pol regulates BV2 cell polarization in vitro. (A) Workflow of vitro experiment. (B) CCK8 assay was used to detect the toxicity of Pol on BV2 cells (n = 5); (C-D) The levels of IL-6 and TNFα mRNA in BV2 cells(n = 4), (E-G)) Levels of Inflammatory cytokine in BV2 cell culture (n = 4–5); (H-I) The levels of M1 type genes CD86, COX-2, (J-K) The levels of M2 type genes CD206, Arg-1 in BV2 cells (n = 4); (L-M) Immunofluorescence detection of BV2 cell polarization representative images and data visualization (n = 4); (N–O) Flow cytometry detection of BV2 cell polarization (n = 4). (P-Q) Apoptosis of neurons(n = 3). Bar= 100 μm. Data are presented as means ± SD, were performed by One-way ANOVA followed by Tukey’s multiple comparisons test. *p < 0.05, **p < 0.01, ****p < 0.0001; ns= no statistical ifference.
Article Snippet: The primary antibody used in this study was: rabbit anti
Techniques: In Vitro, CCK-8 Assay, Cell Culture, Immunofluorescence, Flow Cytometry